Affinity chromatography of viral DNA polymerases on pyran-sepharose.
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چکیده
منابع مشابه
Affinity chromatography of viral DNA polymerases on pyran-sepharose.
Pyran covalently linked to cyanogen bromide-activated Sepharose has been shown to be an effective affinity matrix for several viral DNA polymerases. Differential salt elution of viral compared with cellular polymerases, as well as substrate elution, suggests the affinity nature for the matrix. Unlike some other affinity systems described, pyran-Sepharose is totally resistant to nuclease digesti...
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Because many viruses are readily amenable to molecular, genetic, and biochemical analyses, and because the replication of certain viruses has been the target of antiviral drug development, a wealth of information has accumulated regarding the structure, functions, properties, and regulation of eukaryotic viral DNA polymerases. Viral DNA polymerases have interesting similarities with eukaryotic ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1975
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.72.3.1142